Metabolic Flux Analysis : Methods and Protocols

Metabolic Flux Analysis : Methods and Protocols


Jens O. Kromer, Lars Keld Nielsen, Lars M. Blank
Hardback | 316 pages
178 x 254 x 22.86mm | 8,349g
Publication date
29 Oct 2014
Humana Press Inc.
Publication City/Country
Totowa, NJ, United States
Edition Statement
2014 ed.
Illustrations note
31 Tables, black and white; 51 Illustrations, color; 19 Illustrations, black and white; XII, 316 p. 70 illus., 51 illus. in color.
Bestsellers rank


Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from 13C experiments and three chapters that summarize some recent key findings. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metabolic Flux Analysis: Methods and Protocols presents protocols that cover a range of relevant organisms currently used in the field, providing a solid basis to anybody interested in the field of metabolic flux analysis.

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